Article
Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D.
Nature biotechnology - 1 Oct 2021
van Ineveld Ravian L, Kleinnijenhuis Michiel, Alieva Maria, de Blank Sam, Barrera Roman Mario, van Vliet Esmée J, Martínez Mir Clara, Johnson Hannah R, Bos Frank L, Heukers Raimond, Chuva de Sousa Lopes Susana M, Drost Jarno, Dekkers Johanna F, Wehrens Ellen J, Rios Anne C
Abstract excerpt
Despite advances in three-dimensional (3D) imaging, it remains challenging to profile all the cells within a large 3D tissue, including the morphology and organization of the many cell types present. Here, we introduce eight-color, multispectral, large-scale single-cell resolution 3D (mLSR-3D) imaging and image analysis software for the parallelized, deep learning-based segmentation of large numbers of single...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.