Article
The clustered regularly interspaced short palindromic repeats/associated proteins system for the induction of gene mutations and phenotypic changes in Bombyx mori.
Acta biochimica et biophysica Sinica - 1 Dec 2016
Song Jia, Che Jiaqian, You Zhengying, Ye Xiaogang, Li Jisheng, Ye Lupeng, Zhang Yuyu, Qian Qiujie, Zhong Boxiong
Abstract excerpt
To probe the general phenomena of gene mutations, Bombyx mori, the lepidopterous model organism, was chosen as the experimental model. To easily detect phenotypic variations, the piggyBac system was utilized to introduce two marker genes into the silkworm, and 23.4% transposition efficiency aided in easily breeding a new strain for the entire experiment. Then, the clustered regularly interspaced short palindromic...
Topics
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified
- Base Sequence
- Bombyx
- CRISPR-Cas Systems
- Insect Proteins
- Mutation
- Phenotype