Article
GTR 2.0: gRNA-tRNA Array and Cas9-NG Based Genome Disruption and Single-Nucleotide Conversion in Saccharomyces cerevisiae.
ACS synthetic biology - 18 Jun 2021
Gong Guiping, Zhang Yueping, Wang Zibai, Liu Luo, Shi Shuobo, Siewers Verena, Yuan Qipeng, Nielsen Jens, Zhang Xu, Liu Zihe
Abstract excerpt
Targeted genome disruptions and single-nucleotide conversions with the CRISPR/Cas system have greatly facilitated the development of gene therapy, basic biological research, and synthetic biology. With vast progress in this field, there are still aspects to be optimized, including the target range, the ability to multiplex, the mutation efficiency and specificity, as well as the requirement of adjusting...
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