Article
HLA-A locus allelic dropout in Sanger sequence-based typing due to the single nucleotide polymorphism of exon 1.
International journal of immunogenetics - 1 Dec 2015
Zhang W, He Y, Wang W, Han Z, He J, Chen N, Dong L, Tao S, He J, Zhu F, Lv H
Abstract excerpt
The DNA-based method is used widely for HLA genotyping in routine work, but some allele may be dropout in the genotyping procedure. Here, we reported a case with HLA-A allele dropout in the Sanger PCR-SBT test. The initial PCR-SBT method with a commercial agent kit was not characterized, and the result of Luminex technology indicated the dropout as a HLA-A*02 allele. Subsequently, the sequences of exons 2-4 were...
Topics
- Alleles
- Base Sequence
- Exons
- HLA-A Antigens
- Humans
- Molecular Typing
- Polymorphism, Single Nucleotide
- Reproducibility of Results
- Sequence Analysis, DNA