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Article

High-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins.

Analytical biochemistry - 1 Oct 2017

Yang Xiaolan, Feng Yiran, Chong Huimin, Wang Deqiang, Hu Xiaolei, Pu Jun, Zhan Chang-Guo, Liao Fei

Abstract excerpt

High-throughput estimation of specific activities of an enzyme and its mutants in a group (enzyme/mutants) in cell lysates via high-throughput assay of their activities and separate immunoturbidimetric assay (ITA) of their proteins was proposed. Pseudomonas aeruginosa arylsulfatase (PAAS) and Bacillus fastidious uricase (BFU) served as two models. ITA employed 0.75 mg of antisera against PAAS or BFU as the...

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PMID: 28526525
DOI: 10.1016/j.ab.2017.05.015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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