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Article

An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily.

Proceedings of the National Academy of Sciences of the United States of America - 16 Feb 2010

van Loo Bert, Jonas Stefanie, Babtie Ann C, Benjdia Alhosna, Berteau Olivier, Hyvönen Marko, Hollfelder Florian

Abstract excerpt

We report a catalytically promiscuous enzyme able to efficiently promote the hydrolysis of six different substrate classes. Originally assigned as a phosphonate monoester hydrolase (PMH) this enzyme exhibits substantial second-order rate accelerations ((k(cat)/K(M))/k(w)), ranging from 10(7) to as high as 10(19), for the hydrolyses of phosphate mono-, di-, and triesters, phosphonate monoesters, sulfate...

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Identifiers

PMID: 20133613
DOI: 10.1073/pnas.0903951107
PMCID: PMC2840280

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