Article
Mutational landscape of antibody variable domains reveals a switch modulating the interdomain conformational dynamics and antigen binding.
Proceedings of the National Academy of Sciences of the United States of America - 24 Jan 2017
Koenig Patrick, Lee Chingwei V, Walters Benjamin T, Janakiraman Vasantharajan, Stinson Jeremy, Patapoff Thomas W, Fuh Germaine
Abstract excerpt
Somatic mutations within the antibody variable domains are critical to the immense capacity of the immune repertoire. Here, via a deep mutational scan, we dissect how mutations at all positions of the variable domains of a high-affinity anti-VEGF antibody G6.31 impact its antigen-binding function. The resulting mutational landscape demonstrates that large portions of antibody variable domain positions are open to...
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