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Article

Primer extension-based method for the generation of a siRNA/miRNA expression vector.

Physiological genomics - 14 Nov 2007

Gou Deming, Zhang Honghao, Baviskar Pradyumna S, Liu Lin

Abstract excerpt

RNA interference (RNAi) has become a powerful technique for studying gene function, biological pathways, and the physiology of diseases. Typically, the RNAi response in mammalian cells is mediated by small interfering RNA (siRNA). The use of synthesized siRNA to silence gene is relatively quick and easy, but it is costly with transient effects. A short hairpin RNA (shRNA) with complementary sense and antisense...

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Identifiers

PMID: 17804605
DOI: 10.1152/physiolgenomics.00005.2007

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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