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Article

Direct analysis of RNA after transfection.

Current protocols in molecular biology - 1 May 2001

Kingston R E

Abstract excerpt

It is possible to transfect mammalian cells with a gene of interest and directly detect the RNA made from that gene 24 to 48 hr later. The ability to analyze the RNA directly allows mutation and functional analysis of an intact gene, as the promoter does not have to be subcloned. This technique i...

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Identifiers

PMID: 18265288
DOI: 10.1002/0471142727.mb0908s36

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-18265288-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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