CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer.

doi:10.1038/s41467-021-21867-0

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Article

CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer.

Nature communications - 19 Mar 2021

Ahmed Musaddeque, Soares Fraser, Xia Ji-Han, Yang Yue, Li Jing, Guo Haiyang, Su Peiran, Tian Yijun, Lee Hyung Joo, Wang Miranda, Akhtar Nayeema, Houlahan Kathleen E, Bosch Almudena, Zhou Stanley, Mazrooei Parisa, Hua Junjie T, Chen Sujun, Petricca Jessica, Zeng Yong, Davies Alastair, Fraser Michael, Quigley David A, Feng Felix Y, Boutros Paul C, Lupien Mathieu, Zoubeidi Amina, Wang Liang, Walsh Martin J, Wang Ting, Ren Shancheng, Wei Gong-Hong, He Housheng Hansen

Abstract excerpt

Prostate cancer (PCa) risk-associated SNPs are enriched in noncoding cis-regulatory elements (rCREs), yet their modi operandi and clinical impact remain elusive. Here, we perform CRISPRi screens of 260 rCREs in PCa cell lines. We find that rCREs harboring high risk SNPs are more essential for cell proliferation and H3K27ac occupancy is a strong indicator of essentiality. We also show that cell-line-specific...

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PMID: 33741908
DOI: 10.1038/s41467-021-21867-0
PMCID: PMC7979745

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