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Article

phiC31 integrase-mediated site-specific recombination in barley.

PloS one - 1 Jan 2012

Kapusi Eszter, Kempe Katja, Rubtsova Myroslava, Kumlehn Jochen, Gils Mario

Abstract excerpt

The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination. We produced transgenic barley plants expressing an active phiC31 integrase and crossed them with transgenic barley plants carrying a target locus for recombination. The target sequence involves a reporter gene encoding green fluorescent protein (GFP), which is...

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Identifiers

PMID: 23024817
DOI: 10.1371/journal.pone.0045353
PMCID: PMC3443236

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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