Automated design of CRISPR prime editors for thousands of human pathogenic variants

Abstract excerpt

Prime editors (PEs) are CRISPR-based genome engineering tools that can introduce precise base-pair edits at specific locations in the genome. These programmable gene editors have been predicted to repair 89% of known human pathogenic variants in the ClinVar database, although these PE constructs do not presently exist. Towards this end, we developed an automated pipeline to correct (therapeutic editing) or introdu...

Topics

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• Base Pairing
• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• DNA
• Gene Editing
• Mutation
• RNA, Guide, CRISPR-Cas Systems

Identifiers and source

Literature Corpus work

2923d35d-acd4-5b0b-82ce-a1e229564802

DOI

10.1101/2020.05.07.083444