Article
Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA.
Nature communications - 8 Feb 2021
Poddar Anustup, Azam Muhammad S, Kayikcioglu Tunc, Bobrovskyy Maksym, Zhang Jichuan, Ma Xiangqian, Labhsetwar Piyush, Fei Jingyi, Singh Digvijay, Luthey-Schulten Zaida, Vanderpool Carin K, Ha Taekjip
Abstract excerpt
Base-pairing interactions mediate many intermolecular target recognition events. Even a single base-pair mismatch can cause a substantial difference in activity but how such changes influence the target search kinetics in vivo is unknown. Here, we use high-throughput sequencing and quantitative super-resolution imaging to probe the mutants of bacterial small RNA, SgrS, and their regulation of ptsG mRNA target....
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