Article
Human Rev1 relies on insert-2 to promote selective binding and accurate replication of stabilized G-quadruplex motifs.
Nucleic acids research - 26 Feb 2021
Ketkar Amit, Smith Lane, Johnson Callie, Richey Alyssa, Berry Makayla, Hartman Jessica H, Maddukuri Leena, Reed Megan R, Gunderson Julie E C, Leung Justin W C, Eoff Robert L
Abstract excerpt
We previously reported that human Rev1 (hRev1) bound to a parallel-stranded G-quadruplex (G4) from the c-MYC promoter with high affinity. We have extended those results to include other G4 motifs, finding that hRev1 exhibited stronger affinity for parallel-stranded G4 than either anti-parallel or hybrid folds. Amino acids in the αE helix of insert-2 were identified as being important for G4 binding. Mutating E466...
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