Article
Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genotyping.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc - 1 Jun 2021
Credle Joel J, Robinson Matthew L, Gunn Jonathan, Monaco Daniel, Sie Brandon, Tchir Alexandra, Hardick Justin, Zheng Xuwen, Shaw-Saliba Kathryn, Rothman Richard E, Eshleman Susan H, Pekosz Andrew, Hansen Kasper, Mostafa Heba, Steinegger Martin, Larman H Benjamin
Abstract excerpt
There is an urgent and unprecedented need for sensitive and high-throughput molecular diagnostic tests to combat the SARS-CoV-2 pandemic. Here we present a generalized version of the RNA-mediated oligonucleotide Annealing Selection and Ligation with next generation DNA sequencing (RASL-seq) assay, called "capture RASL-seq" (cRASL-seq), which enables highly sensitive (down to ~1-100 pfu/ml or cfu/ml) and highly...
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