Article

ApharSeq: An Extraction-free Early-Pooling Protocol for Massively Multiplexed SARS-CoV-2 Detection

2020-08-13

Abstract excerpt

The global SARS-CoV-2 pandemic created a dire need for viral detection tests worldwide. Most current tests for SARS-CoV-2 are based on RNA extraction followed by quantitative reverse-transcription PCR assays. While automation and improved logistics increased the capacity of these tests, they cannot exceed the lower bound dictated by one extraction and one RT-PCR reaction per sample. Multiplexed next generation seq...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 64c2df7b-a723-59a5-a14f-2b96d488ad61
DOI: 10.1101/2020.08.08.20170746

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

ApharSeq: An Extraction-free Early-Pooling Protocol for Massively Multiplexed SARS-CoV-2 DetectionDOI 10.1101/2020.08.08.20170746Read

One-Seq: A Highly Scalable Sequencing-Based Diagnostic for SARS-CoV-2 and Other Single-Stranded VirusesSemantic proximity Read

Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genotypingSemantic proximity Read

HiDRA-seq: High-Throughput SARS-CoV-2 Detection by RNA Barcoding and Amplicon SequencingSemantic proximity Read

Swab-Seq: A high-throughput platform for massively scaled up SARS-CoV-2 testingSemantic proximity Read

McQ – An open-source multiplexed SARS-CoV-2 quantification platformSemantic proximity Read

Rapid High-Throughput Whole-Genome Sequencing of SARS-CoV-2 by Using One-Step Reverse Transcription-PCR Amplification with an Integrated Microfluidic System and Next-Generation SequencingSemantic proximity Read

Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genotyping.Semantic proximity Read

Microfluidic Nano-Scale qPCR Enables Ultra-Sensitive and Quantitative Detection of SARS-CoV-2Semantic proximity Read

Rapid High Throughput Whole Genome Sequencing of SARS-CoV-2 by using One-step RT-PCR Amplification with Integrated Microfluidic System and Next-Gen SequencingSemantic proximity Read

Flexible upscaling of laboratory PCR testing capacity at the Robert Koch Institute during the SARS-CoV-2 pandemicSemantic proximity Read

A Multiplexed, Next Generation Sequencing Platform for High-Throughput Detection of SARS-CoV-2Semantic proximity Read

A streamlined approach to rapidly detect SARS-CoV-2 infection, avoiding RNA purificationSemantic proximity Read