Article
Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals.
PloS one - 1 Jan 2021
Doddapaneni Harsha, Cregeen Sara Javornik, Sucgang Richard, Meng Qingchang, Qin Xiang, Avadhanula Vasanthi, Chao Hsu, Menon Vipin, Nicholson Erin, Henke David, Piedra Felipe-Andres, Rajan Anubama, Momin Zeineen, Kottapalli Kavya, Hoffman Kristi L, Sedlazeck Fritz J, Metcalf Ginger, Piedra Pedro A, Muzny Donna M, Petrosino Joseph F, Gibbs Richard A
Abstract excerpt
The newly emerged and rapidly spreading SARS-CoV-2 causes coronavirus disease 2019 (COVID-19). To facilitate a deeper understanding of the viral biology we developed a capture sequencing methodology to generate SARS-CoV-2 genomic and transcriptome sequences from infected patients. We utilized an oligonucleotide probe-set representing the full-length genome to obtain both genomic and transcriptome (subgenomic open...
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