Article
Co-transcriptional splicing regulates 3' end cleavage during mammalian erythropoiesis.
Molecular cell - 4 Mar 2021
Reimer Kirsten A, Mimoso Claudia A, Adelman Karen, Neugebauer Karla M
Abstract excerpt
Pre-mRNA processing steps are tightly coordinated with transcription in many organisms. To determine how co-transcriptional splicing is integrated with transcription elongation and 3' end formation in mammalian cells, we performed long-read sequencing of individual nascent RNAs and precision run-on sequencing (PRO-seq) during mouse erythropoiesis. Splicing was not accompanied by transcriptional pausing and was...
Topics
- Animals
- Base Sequence
- Cell Differentiation
- Cell Line, Tumor
- Erythroid Cells
- Erythropoiesis
- Exons
- Humans
- Introns
- Leukocytes
- Mice
- Mutation
- RNA Cleavage
- RNA Polymerase II
- RNA Splice Sites