Article
Simultaneous site-directed mutagenesis of duplicated loci in soybean using a single guide RNA.
Plant cell reports - 1 Mar 2018
Kanazashi Yuhei, Hirose Aya, Takahashi Ippei, Mikami Masafumi, Endo Masaki, Hirose Sakiko, Toki Seiichi, Kaga Akito, Naito Ken, Ishimoto Masao, Abe Jun, Yamada Tetsuya
Abstract excerpt
KEY MESSAGE: Using a gRNA and Agrobacterium-mediated transformation, we performed simultaneous site-directed mutagenesis of two GmPPD loci in soybean. Mutations in GmPPD loci were confirmed in at least 33% of T2 seeds. The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated endonuclease 9 (Cas9) system is a powerful tool for site-directed mutagenesis in crops. Using a single guide...
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