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Article

An efficient transgene-free DNA-editing system for Arabidopsis using a fluorescent marker.

Biotechnology letters - 1 Feb 2020

Ouyang Lejun, Ma Mingsai, Li Limei

Abstract excerpt

OBJECTIVE: To obtain transgene-free progeny by constructing a DNA editing system with a fluorescent screening marker gene and two pairs of single-guide RNAs to simultaneously recognize two different sites in the target gene encoding Arabidopsis microRNA(miR)160A RESULTS: The T1 seeds with red fluorescence were easily identified and were selected to verify that the proportion of miR160A knockout mutants reached...

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PMID: 31797132
DOI: 10.1007/s10529-019-02778-z

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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