Article
Construction of a plasmid vector for the regulatable high level expression of eukaryotic genes in Escherichia coli: an application to overproduction of chicken lysozyme.
Protein engineering - 1 Jan 2000
Miki T, Yasukochi T, Nagatani H, Furuno M, Orita T, Yamada H, Imoto T, Horiuchi T
Abstract excerpt
A novel expression vector pKP1500 for synthesizing unfused protein in Escherichia coli was constructed. pKP1500 perserves the tac promoter, the lacZ SD sequence, unique restriction sites (EcoRI, SmaI, BamHI, SalI, PstI and HindIII) and the rrnB terminators of pKK223-3, but the replication origin is replaced with that of pUC9. Construction of this plasmid is based upon the observation that the copy number control...
Topics
- Animals
- Base Sequence
- Chickens
- Culture Media
- DNA
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression Regulation
- Genes, Bacterial
- Genetic Vectors