Article
High-level expression of Bacillus subtilis tryptophanyl-tRNA synthetase in Escherichia coli.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Feb 1990
Shi W, Chow K C, Wong J T
Abstract excerpt
The trpS gene encoding Bacillus subtilis tryptophanyl-tRNA synthetase (TrpRS) was prepared from the pUC8-derived pTSQ2 plasmid, mutagenized to introduce an EcoRI site immediately in front of the ATG start codon, and inserted into the pKK223-3 vector downstream to the tac promoter to yield the pKS...
Topics
- Amino Acyl-tRNA Synthetases
- Bacillus subtilis
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Genes, Bacterial
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Tryptophan-tRNA Ligase