Article
A fast and robust iterative genome-editing method based on a Rock-Paper-Scissors strategy.
Nucleic acids research - 25 Jan 2021
Wang Jichao, Sui Xinyue, Ding Yamei, Fu Yingxin, Feng Xinjun, Liu Min, Zhang Youming, Xian Mo, Zhao Guang
Abstract excerpt
The production of optimized strains of a specific phenotype requires the construction and testing of a large number of genome modifications and combinations thereof. Most bacterial iterative genome-editing methods include essential steps to eliminate selection markers, or to cure plasmids. Additionally, the presence of escapers leads to time-consuming separate single clone picking and subsequent cultivation...
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