Article
Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations.
PloS one - 1 Jan 2020
Kato-Minoura Takako, Ogiwara Yutaro, Yamano Takashi, Fukuzawa Hideya, Kamiya Ritsu
Abstract excerpt
The single-cell green alga Chlamydomonas reinhardtii possesses two α-tubulin genes (tua1 and tua2) and two β-tubulin genes (tub1 and tub2), with the two genes in each pair encoding identical amino acid sequences. Here, we screened an insertional library to establish eight disruptants with defective tua2, tub1, or tub2 expression. Most of the disruptants did not exhibit major defects in cell growth, flagellar...
Topics
- Chlamydomonas reinhardtii
- Genes, Plant
- Mutation
- Plant Proteins
- Tubulin