Article
mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging.
Proceedings of the National Academy of Sciences of the United States of America - 1 Dec 2020
Campbell Benjamin C, Nabel Elisa M, Murdock Mitchell H, Lao-Peregrin Cristina, Tsoulfas Pantelis, Blackmore Murray G, Lee Francis S, Liston Conor, Morishita Hirofumi, Petsko Gregory A
Abstract excerpt
Although ubiquitous in biological studies, the enhanced green and yellow fluorescent proteins (EGFP and EYFP) were not specifically optimized for neuroscience, and their underwhelming brightness and slow expression in brain tissue limits the fidelity of dendritic spine analysis and other indispensable techniques for studying neurodevelopment and plasticity. We hypothesized that EGFP's low solubility in mammalian...
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