Article
Highly sensitive and quantitative FRET-FLIM imaging in single dendritic spines using improved non-radiative YFP.
Brain cell biology - 1 Aug 2008
Murakoshi Hideji, Lee Seok-Jin, Yasuda Ryohei
Abstract excerpt
Two-photon fluorescence lifetime imaging microscopy (TPFLIM) enables the quantitative measurements of fluorescence resonance energy transfer (FRET) in small subcellular compartments in light scattering tissue. We evaluated and optimized the FRET pair of mEGFP (monomeric EGFP with the A206K mutati...
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