Article
A Novel Ultra-Stable, Monomeric Green Fluorescent Protein For Direct Volumetric Imaging of Whole Organs Using CLARITY.
Scientific reports - 12 Jan 2018
Scott Daniel J, Gunn Natalie J, Yong Kelvin J, Wimmer Verena C, Veldhuis Nicholas A, Challis Leesa M, Haidar Mouna, Petrou Steven, Bathgate Ross A D, Griffin Michael D W
Abstract excerpt
Recent advances in thick tissue clearing are enabling high resolution, volumetric fluorescence imaging of complex cellular networks. Fluorescent proteins (FPs) such as GFP, however, can be inactivated by the denaturing chemicals used to remove lipids in some tissue clearing methods. Here, we solved the crystal structure of a recently engineered ultra-stable GFP (usGFP) and propose that the two stabilising...
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