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The RNA Polymerase α Subunit Recognizes the DNA Shape of the Upstream Promoter Element.

Biochemistry - 8 Dec 2020

Lara-Gonzalez Samuel, Dantas Machado Ana Carolina, Rao Satyanarayan, Napoli Andrew A, Birktoft Jens, Di Felice Rosa, Rohs Remo, Lawson Catherine L

Abstract excerpt

We demonstrate here that the α subunit C-terminal domain of Escherichia coli RNA polymerase (αCTD) recognizes the upstream promoter (UP) DNA element via its characteristic minor groove shape and electrostatic potential. In two compositionally distinct crystallized assemblies, a pair of αCTD subunits bind in tandem to the UP element consensus A-tract that is 6 bp in length (A6-tract), each with their arginine 265...

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Identifiers

PMID: 33205945
DOI: 10.1021/acs.biochem.0c00571
PMCID: PMC8100869

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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