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Article

PIK3CA mutation enrichment and quantitation from blood and tissue.

Scientific reports - 13 Oct 2020

Keraite Ieva, Alvarez-Garcia Virginia, Garcia-Murillas Isaac, Beaney Matthew, Turner Nicholas C, Bartos Clare, Oikonomidou Olga, Kersaudy-Kerhoas Maïwenn, Leslie Nicholas R

Abstract excerpt

PIK3CA is one of the two most frequently mutated genes in breast cancers, occurring in 30-40% of cases. Four frequent 'hotspot' PIK3CA mutations (E542K, E545K, H1047R and H1047L) account for 80-90% of all PIK3CA mutations in human malignancies and represent predictive biomarkers. Here we describe a PIK3CA mutation specific nuclease-based enrichment assay, which combined with a low-cost real-time qPCR detection...

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Identifiers

PMID: 33051521
DOI: 10.1038/s41598-020-74086-w
PMCID: PMC7555501

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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