Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Highly Sensitive Detection of PIK3CA Mutations by Looping-Out Probes-Based Melting Curve Analysis.

Biochemical genetics - 1 Feb 2024

Xu Boheng, Lan Yanping, Luo Danjiao, Zheng Yangsi, Ni Runfang, Su Guoqiang, Huang Qiuying, Li Qingge

Abstract excerpt

PIK3CA mutations have important therapeutic and prognostic implications in various cancer types. However, highly sensitive detection of PIK3CA hotspot mutations in heterogeneous tumor samples remains a challenge in clinical settings. To establish a rapid PCR assay for highly sensitive detection of multiple PIK3CA hotspot mutations. We described a novel melting curve analysis-based assay using looping-out probes...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Class I Phosphatidylinositol 3-Kinases
Share to DNA Mutational Analysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation

Share to Neoplasms

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 37249716
DOI: 10.1007/s10528-023-10408-7

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Highly Sensitive Detection of PIK3CA Mutations by Looping-Out Probes-Based Melting Curve Analysis.PMID 37249716Read ↗

PIK3CA mutation enrichment and quantitation from blood and tissue.Semantic proximity Read ↗

PIK3CA hotspot mutation scanning by a novel and highly sensitive high-resolution small amplicon melting analysis method.Semantic proximity Read ↗

Dual-mode temperature-switchable TSCP probes for precise analysis of PIK3CA mutations and VAF.Semantic proximity Read ↗

Accurate Detection of Multiple Tumor Mutations in Formalin-Fixed Paraffin-Embedded Tissues by Coupling Sequence Artifacts Elimination and Mutation Enrichment With MeltArray.Semantic proximity Read ↗

A simple and robust real-time qPCR method for the detection of PIK3CA mutations.Semantic proximity Read ↗

PIK3CA mutation profiling in patients with breast cancer, using a highly sensitive detection system.Semantic proximity Read ↗

Development of multiplex digital PCR assays for the detection of PIK3CA mutations in the plasma of metastatic breast cancer patients.Semantic proximity Read ↗

High-resolution melting analysis as a sensitive prescreening diagnostic tool to detect KRAS , BRAF , PIK3CA , and AKT1 mutations in formalin-fixed, paraffin-embedded tissues.Semantic proximity Read ↗

A Multiplex Assay for Fast PIK3CA Hotspot Mutation Characterization in a Single Specimen by 3-Color Digital PCR Analysis.Semantic proximity Read ↗

Direct comparison of an ultrasensitive real-time PCR assay with droplet digital PCR for the detection of PIK3CA hotspot mutations in primary tumors, plasma cell-free DNA and paired CTC-derived gDNAsSemantic proximity Read ↗

A droplet digital LAMP-based lab-on-a-disc system for multiplex allele-specific detection of tumor-derived DNA mutations.Semantic proximity Read ↗

A SNaPshot assay for the rapid and simple detection of four common hotspot codon mutations in the PIK3CA geneSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.