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Article

A Multiplex Assay for Fast PIK3CA Hotspot Mutation Characterization in a Single Specimen by 3-Color Digital PCR Analysis.

The journal of applied laboratory medicine - 3 Sept 2024

Helmijr Jean, Motta Gianmarco, Jongbloed Lisa, de Weerd Vanja, van Bergen Lotte, Verschoor Noortje, Stella Stefania, Beaufort Corine, Vigneri Paolo, Martens John W M, Wilting Saskia M, Jansen Maurice P H M

Abstract excerpt

BACKGROUND: Activating mutations in the phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) gene have been detected often in solid tumors. Targeted therapy for mutant PIK3CA is now available in the clinic, making molecular diagnostics pivotal. Our aim was to design a multiplex digital PCR (dPCR) assay to evaluate the 4 most common PIK3CA hotspot mutations simultaneously to characterize...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 39012846
DOI: 10.1093/jalm/jfae064

Genes

PIK3CA

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

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