Article
Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation.
PLoS biology - 1 Oct 2020
Kobia Francis M, Preusse Kristina, Dai Quanhui, Weaver Nicholas, Hass Matthew R, Chaturvedi Praneet, Stein Sarah J, Pear Warren S, Yuan Zhenyu, Kovall Rhett A, Kuang Yi, Eafergen Natanel, Sprinzak David, Gebelein Brian, Brunskill Eric W, Kopan Raphael
Abstract excerpt
Cooperative DNA binding is a key feature of transcriptional regulation. Here we examined the role of cooperativity in Notch signaling by CRISPR-mediated engineering of mice in which neither Notch1 nor Notch2 can homo- or heterodimerize, essential for cooperative binding to sequence-paired sites (SPS) located near many Notch-regulated genes. Although most known Notch-dependent phenotypes were unaffected in...
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