Article
Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.
Developmental cell - 12 Jan 2015
Kok F O, Shin M, Ni C-W, Gupta A, Grosse A S, van Impel A, Kirchmaier B C, Peterson-Maduro J, Kourkoulis G, Male I, DeSantis D F, Sheppard-Tindell S, Ebarasi L, Betsholtz C, Schulte-Merker S, Wolfe S A, Lawson N D
Abstract excerpt
The widespread availability of programmable site-specific nucleases now enables targeted gene disruption in the zebrafish. In this study, we applied site-specific nucleases to generate zebrafish lines bearing individual mutations in more than 20 genes. We found that mutations in only a small proportion of genes caused defects in embryogenesis. Moreover, mutants for ten different genes failed to recapitulate...
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