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Article

The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2.

Journal of virology - 9 Nov 2020

Ogando Natacha S, Zevenhoven-Dobbe Jessika C, van der Meer Yvonne, Bredenbeek Peter J, Posthuma Clara C, Snijder Eric J

Abstract excerpt

Coronaviruses (CoVs) stand out for their large RNA genome and complex RNA-synthesizing machinery comprising 16 nonstructural proteins (nsps). The bifunctional nsp14 contains 3'-to-5' exoribonuclease (ExoN) and guanine-N7-methyltransferase (N7-MTase) domains. While the latter presumably supports mRNA capping, ExoN is thought to mediate proofreading during genome replication. In line with such a role, ExoN knockout...

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Identifiers

PMID: 32938769
DOI: 10.1128/JVI.01246-20
PMCID: PMC7654266

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