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Article

Recovery of proofreading-impaired SARS-CoV-2 reveals a mutator phenotype and an ExoN activity threshold for viability.

Journal of virology - 18 Aug 2026

He Li, Su Yuan-Wei Norman, Zhang Fushun, Moustafa Ibrahim M, Gohara David W, Ye Chengjin, Martinez-Sobrido Luis, Arnold Jamie J, Cameron Craig E, Xiang Yan

Abstract excerpt

Coronaviruses (CoVs) replicate unusually large RNA genomes that necessitate proofreading by the 3'-to-5' exoribonuclease (ExoN) formed by nonstructural proteins 14 (nsp14) and 10 (nsp10). Previous studies suggested that inactivation of the ExoN catalytic site in severe acute respiratory syndrome CoV 2 (SARS-CoV-2) is lethal, leaving unresolved whether the virus can tolerate impaired proofreading activity. Here,...

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Chlorocebus aethiops
COVID-19
Exoribonucleases
Genome, Viral
SARS-CoV-2

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Viral Nonstructural Proteins

Virus Replication

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Identifiers

PMID: 42524883
DOI: 10.1128/jvi.00809-26
PMCID: PMC13483246

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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