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Article

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.

Scientific reports - 27 Nov 2013

Mashiko Daisuke, Fujihara Yoshitaka, Satouh Yuhkoh, Miyata Haruhiko, Isotani Ayako, Ikawa Masahito

Abstract excerpt

CRISPR/Cas mediated genome editing has been successfully demonstrated in mammalian cells and further applications for generating mutant mice were reported by injecting humanized Cas9 (hCas) mRNA and single guide RNA into fertilized eggs. Here we inject the circular plasmids expressing hCas9 and sgRNA into mouse zygotes and obtained mutant mice within a month. When we targeted the Cetn1 locus, 58.8% (10/17) of the...

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Identifiers

PMID: 24284873
DOI: 10.1038/srep03355
PMCID: PMC3842082

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Source receipt: pubmed, retrieved 7 Sept 2026.

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