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Article

Increasing the synthetic performance of penicillin acylase PAS2 by structure-inspired semi-random mutagenesis.

Protein engineering, design & selection : PEDS - 1 Jul 2004

Gabor Esther M, Janssen Dick B

Abstract excerpt

A semi-random mutagenesis approach was followed to increase the performance of penicillin acylase PAS2 in the kinetically controlled synthesis of ampicillin from 6-aminopenicillanic acid (6-APA) and activated D-phenylglycine derivatives. We directed changes in amino acid residues to positions close to the active site that are expected to affect the catalytic performance of penicillin acylase: alpha R160, alpha...

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PMID: 15333773
DOI: 10.1093/protein/gzh070

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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