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Generating combinatorial diversity via engineered V(D)J-like recombination in Saccharomyces cerevisiae

1 Jul 2025

Abstract excerpt

V(D)J recombination is integral to the development of antibody diversity and proceeds through a complex DNA cleavage and repair process mediated by several proteins, including recombination-activating genes 1 and 2, RAG1 and RAG2. V(D)J recombination occurs in all jawed vertebrates but is absent from evolutionarily distant relatives, including the yeast Saccharomyces cerevisiae. As yeast grow quickly and are a...

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PMID: 40592886
DOI: 10.1038/s41467-025-61206-1

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