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Article

Optimization of multiplexed CRISPR/Cas9 system for highly efficient genome editing in Setaria viridis.

The Plant journal : for cell and molecular biology - 1 Nov 2020

Weiss Trevor, Wang Chunfang, Kang Xiaojun, Zhao Hui, Elena Gamo Maria, Starker Colby G, Crisp Peter A, Zhou Peng, Springer Nathan M, Voytas Daniel F, Zhang Feng

Abstract excerpt

In recent years, Setaria viridis has been developed as a model plant to better understand the C4 photosynthetic pathway in major crops. With the increasing availability of genomic resources for S. viridis research, highly efficient genome editing technologies are needed to create genetic variation resources for functional genomics. Here, we developed a protoplast assay to rapidly optimize the multiplexed...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32786122
DOI: 10.1111/tpj.14949

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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