Article
CRISPR/Cas9-mediated efficient targeted mutagenesis in grape in the first generation.
Plant biotechnology journal - 1 Apr 2018
Wang Xianhang, Tu Mingxing, Wang Dejun, Liu Jianwei, Li Yajuan, Li Zhi, Wang Yuejin, Wang Xiping
Abstract excerpt
The clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR/Cas9) system is a powerful tool for editing plant genomes. Efficient genome editing of grape (Vitis vinifera) suspension cells using the type II CRISPR/Cas9 system has been demonstrated; however, it has not been established whether this system can be applied to get biallelic mutations in the first generation of grape. In...
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