Article

Cas12a/3 crRNAs RNP transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome in the T0 generation

2024-06-17

Abstract excerpt

Citrus canker, caused by Xanthomonas citri subsp. citri ( Xcc ), is a devastating disease worldwide. Previously, we successfully generated canker-resistant Citrus sinensis cv. Hamlin lines in the T0 generation, achieving a mutation efficiency of 97.4%. This was achieved through the transformation of embryogenic protoplasts using the Cas12a/1 crRNA ribonucleoprotein (RNP) system to edit the canker susceptibility...

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Literature Corpus work: e46cb9d7-c290-5ac8-9509-382f7acfb82a
DOI: 10.1101/2024.06.13.598908

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Cas12a/3 crRNAs RNP transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome in the T0 generationDOI 10.1101/2024.06.13.598908Read

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