Article
The In Situ Structure of Parkinson's Disease-Linked LRRK2.
Cell - 17 Sept 2020
Watanabe Reika, Buschauer Robert, Böhning Jan, Audagnotto Martina, Lasker Keren, Lu Tsan-Wen, Boassa Daniela, Taylor Susan, Villa Elizabeth
Abstract excerpt
Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most frequent cause of familial Parkinson's disease. LRRK2 is a multi-domain protein containing a kinase and GTPase. Using correlative light and electron microscopy, in situ cryo-electron tomography, and subtomogram analysis, we reveal a 1...
Topics
- Cryoelectron Microscopy
- Cytoplasm
- Electron Microscope Tomography
- GTP Phosphohydrolases
- HEK293 Cells
- Humans
- Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
- Microscopy, Electron, Transmission
- Microtubules
- Models, Chemical
- Mutation