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Article

Direct analysis of brain phenotypes via neural blastocyst complementation.

Nature protocols - 1 Oct 2020

Dai Hai-Qiang, Liang Zhuoyi, Chang Amelia N, Chapdelaine-Williams Aimee M, Alvarado Beatriz, Pollen Alex A, Alt Frederick W, Schwer Bjoern

Abstract excerpt

We provide a protocol for generating forebrain structures in vivo from mouse embryonic stem cells (ESCs) via neural blastocyst complementation (NBC). We developed this protocol for studies of development and function of specific forebrain regions, including the cerebral cortex and hippocampus. We describe a complete workflow, from methods for modifying a given genomic locus in ESCs via CRISPR-Cas9-mediated...

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Identifiers

PMID: 32778838
DOI: 10.1038/s41596-020-0364-y
PMCID: PMC7685531

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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