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Article

Monitoring ligand-mediated helix 12 transitions within the human estrogen receptor α using bipartite tetracysteine display.

Organic & biomolecular chemistry - 21 Aug 2020

Pokhrel Ranju, Tang Tang, Holub Justin M

Abstract excerpt

Estrogen receptor α ligand-binding domains (ERα-LBD) expressing tetracysteine motifs bind FlAsH-EDT2 upon transition of helix 12 (H12) to a folded state. Changes in fluorescence intensity allowed surveillance of ligand-mediated H12 transitions and facilitated the determination of FlAsH association rates (kon) and apparent equilibrium dissociation constants (Kapp) to ERα-LBDs in the presence of estrogenic ligands.

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Identifiers

PMID: 32724950
DOI: 10.1039/d0ob01234c

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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