Simulating ComBat: how batch correction can lead to the systematic introduction of false positive results in DNA methylation microarray studies

Abstract excerpt

BACKGROUND: Systematic technical effects-also called batch effects-are a considerable challenge when analyzing DNA methylation (DNAm) microarray data, because they can lead to false results when confounded with the variable of interest. Methods to correct these batch effects are error-prone, as previous findings have shown. RESULTS: Here, we demonstrate how using the R function ComBat to correct simulated...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Epigenetics and DNA Methylation
• Share to Gene expression and cancer classification
• Share to Prenatal Screening and Diagnostics