Universal Southern blot protocol with cold or radioactive probes for the validation of alleles obtained by homologous recombination.

doi:10.1016/j.ymeth.2020.06.011

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Article

Universal Southern blot protocol with cold or radioactive probes for the validation of alleles obtained by homologous recombination.

Methods (San Diego, Calif.) - 1 Jul 2021

Codner Gemma F, Erbs Valerie, Loeffler Jorik, Chessum Lauren, Caulder Adam, Jullien Nicolas, Wells Sara, Birling Marie-Christine, Teboul Lydia

Abstract excerpt

The widespread availability of recombineered vectors and gene targeted embryonic stem cells from large-scale repositories facilitates the generation of mouse models for functional genetic studies. Southern blotting validates the structure of these targeted alleles produced by homologous recombination, as well as indicating any additional integrations of the vector into the genome. Traditionally this technique...

Topics

Alleles
Animals
Blotting, Southern
Gene Targeting
Genetic Vectors
Homologous Recombination
Mice
Radioactivity

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Identifiers

PMID: 32599056
DOI: 10.1016/j.ymeth.2020.06.011
PMCID: PMC10790599

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