Article
Recycling of ribosomal complexes stalled at the step of elongation in Escherichia coli.
Journal of molecular biology - 11 Jul 2008
Singh Nongmaithem Sadananda, Ahmad Rais, Sangeetha Ramachandran, Varshney Umesh
Abstract excerpt
Translating ribosomes often stall during elongation. The stalled ribosomes are known to be recycled by tmRNA (SsrA)-mediated trans-translation. Another process that recycles the stalled ribosomes is characterized by peptidyl-tRNA release. However, the mechanism of peptidyl-tRNA release from the stalled ribosomes is not well understood. We used a defined system of an AGA-minigene containing a small open reading...
Topics
- Alleles
- Escherichia coli
- Plasmids
- Prokaryotic Initiation Factor-3
- RNA, Bacterial
- RNA, Transfer, Amino Acyl
- Ribosomal Proteins
- Ribosomes
