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Article

Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Smith Katherine A, Prantzalos Emily R, Anantharam Arun

Abstract excerpt

Of the techniques currently available to monitor dense core granule exocytosis in adrenal chromaffin cells, two have proven particularly useful: carbon-fiber amperometry and total internal reflection fluorescence (TIRF) microscopy. Amperometry enables the detection of oxidizable catecholamines escaping a fusion pore with millisecond time resolution. TIRF microscopy, and its variant polarized-TIRF (pTIRF)...

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Identifiers

PMID: 32529370
DOI: 10.1007/978-1-0716-0676-6_12
PMCID: PMC11637319

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