Article
Recognition of nuclear export signals by CRM1 carrying the oncogenic E571K mutation.
Molecular biology of the cell - 1 Aug 2020
Baumhardt Jordan M, Walker Janek S, Lee Yoonji, Shakya Binita, Brautigam Chad A, Lapalombella Rosa, Grishin Nick, Chook Yuh Min
Abstract excerpt
The E571K mutation of CRM1 is highly prevalent in some cancers, but its mechanism of tumorigenesis is unclear. Glu571 of CRM1 is located in its nuclear export signal (NES)-binding groove, suggesting that binding of select NESs may be altered. We generated HEK 293 cells with either monoallelic CRM1WT/E571K or biallelic CRM1E571K/E571K using CRISPR/Cas9. We also combined analysis of binding affinities and...
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