Article
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing.
Journal of visualized experiments : JoVE - 21 May 2020
Zhang Jesse Q, Chang Kai-Chun, Liu Leqian, Gartner Zev J, Abate Adam R
Abstract excerpt
The powerful tools available to edit yeast genomes have made this microbe a valuable platform for engineering. While it is now possible to construct libraries of millions of genetically distinct strains, screening for a desired phenotype remains a significant obstacle. With existing screening techniques, there is a tradeoff between information output and throughput, with high-throughput screening typically being...
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